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Sample collection and testing scheme. UVT samples were aliquoted for use in all three HSV assays (Elvis culture, PCR, and HSVQx). HSV-1 testing in the Elvis system could be performed only on HSV-2-negative samples. The sample collected using the assay collection kit (Qx WS) and the UVT aliquoted into the collection kit were both tested on the HSVQx system. +, positive; −, negative.
