Fig 10.

Proposed model of DNAJC14 action and RC formation. Viral proteins (green) and DNAJC14 (red) are targeted to DRMs within the ER membrane. Membrane targeting of DNAJC14 can be mediated by its TM or MBD. Viral infection prompts the clustering of these DRMs, which in turn facilitates protein-protein interactions between the viral proteins and between a viral protein(s) and DNAJC14, allowing chaperone events that facilitate RC formation to occur. The RC, once formed, is maintained by a protein scaffold consisting of viral proteins and possibly host proteins and also contains the viral dsRNA replication intermediate. The RC protein scaffold remains intact after Triton solubilization and fails to float in membrane flotation assays, while viral NS proteins that failed to undergo the chaperone process remain in the DRMs after Triton solubilization and float in membrane flotation assays.