Fig 2.

Dietary intervention with scGOS/lcFOS/pAOS (GFA) decreases the Th2 type immune response and lung eosinophilia upon challenge in FI-RSV-vaccinated mice. (A) Mice received prebiotic or control diet starting 2 weeks before i.m. vaccination with 50 μl of FI-RSV. After RSV challenge at day 35 after vaccination, mice were sacrificed at the indicated time points. (B) Cell composition of BAL fluid (left, total cell numbers; right, indicated cell type as a percentage of total BAL cells). Percentages of eosinophils were significantly decreased in mice treated with the GFA diet (P < 0.05). (C and D) Percentages of IFN-γ-, IL-4-, IL-5-, and IL-13-producing cells of total CD4⁺ T cells and total IFN-γ-, IL-4-, IL-5-, and IL-13-producing CD4⁺ T cell numbers after in vitro restimulation of lung CD4⁺ T cells with unloaded (D1) or RSV-loaded (D1+RSV) myeloid dendritic cells. Data shown represent the means ± SEM from 2 individual experiments performed with similar results, n = 8/group.