Fig 4.

Dietary intervention with scGOS/lcFOS/pAOS (GFA) does not affect body weight but has a significant effect on viral clearance in FI-RSV-vaccinated mice. (A) Daily weight measurements were performed for 8 days after RSV infection. The percentage of original body weight is shown. (B) Viral replication in lungs of mice was measured by plaque assay and shown as PFU/gram of lung (left). Amounts of RSV A2 present in lung of FI-RSV-vaccinated mice were measured by RT-PCR. Concentrations of RSV-N RNA were determined by comparing sample threshold values to a standard curve of RSV A2 and are shown as copies of RSV-N/gram of lung (right). (C) Development of the RSV-specific CD8⁺ T cell response measured as H-2D^b/M_187-195 tetramer-positive cells as a percentage of total CD8⁺ T cells (left) and absolute numbers (right) in BAL fluid. Kinetics of eosinophil (CD45⁺ CD11c⁻ Siglec-F⁺) influx in BAL fluid as a percentage of total BAL cells are shown (left) and are depicted as absolute numbers (right). (D) Kinetics of CD4⁺ Foxp3⁺ regulatory T cells in BAL fluid of FI-RSV-vaccinated mice at days 4, 6, and 8 after infection (left) and GzmB expression in CD4⁺ Foxp3⁺ cells (right) at these time points. Data shown represent the means ± SEM, n = 5/group. Significant differences are indicated with an asterisk (P < 0.05).