Table 2.
Dietary intervention with scGOS/lcFOS/pAOS increases RSV-specific lung CD8+ T cell response in FI-RSV vaccinated micea
| Day | T cell responseb |
|||||||
|---|---|---|---|---|---|---|---|---|
| % NKG2A+/CD8+ |
% IFNγ+/CD8+ (D1-RSV) |
%TET+/CD8+ |
% IFNγ+/CD8+ (NAITNAKII) |
|||||
| Ctrl | GFA | Ctrl | GFA | Ctrl | GFA | Ctrl | GFA | |
| 4 | 19.1 ± 1.5 | 17.4 ± 0.8 | 6.2 ± 0.6 | 5.2 ± 0.2 | 1.5 ± 0.3 | 1.3 ± 0.2 | 4.7 ± 0.5 | 3.4 ± 0.2 |
| 6 | 22.7 ± 2.1 | 25.0 ± 1.9 | 13.0 ± 1.2 | 14.1 ± 1.3 | 5.1 ± 0.5 | 6.8 ± 0.9 | 6.3 ± 0.8 | 8.3 ± 1.4 |
| 8 | 47.6 ± 4.7 | 52.4 ± 4.7 | 26.1 ± 2.0 | 29.6 ± 3.0 | 12.8 ± 2.6 | 18.3 ± 2.6* | 13.8 ± 2.5 | 19.7 ± 2.8* |
C57BL/6 mice received scGOS/lcFOS/pAOS (GFA) or control diet starting 2 weeks before i.m. vaccination with 50 μl of FI-RSV. After 35 days, mice were i.n. challenged with 2.0 × 106 PFU RSV. At the indicated time points, lymphocytes were isolated from the lung parenchyma and stained for CD8 in combination with NKG2A or H-2Db/M187-195 tetramer. For in vitro T cell restimulation experiments, lung cells were stimulated with RSV-infected D1 cells or the RSV epitope NAITNAKII and intracellularly stained for IFN-γ.
The values depicted represent the number of NKG2A+/CD8+, IFN-γ+/CD8+, or TET+/CD8+ double positive cells as percentages of total CD8+ T cells. The table shows the means ± SEM of control or GFA-treated mice from 2 individual experiments, n = 8/group. Values denoted with an asterisk represent a significant (P < 0.05) increase in NAITNAKII-specific (IFN-γ-producing) CD8+ T cells.