Figure 2.
Effect of alkaline treatment on in vitro [γ-32P]ATP-labeled proteins of soluble fractions from coconut zygotic embryos of developmental stages 11 and 15. [32P]-Labeled proteins were separated by 10% SDS-PAGE and electroblotted onto nylon membranes. Membranes were left without treatment (A) or treated with 1 m KOH (B), and bands were visualized by autoradiography. Figures with arrowheads on the left show the molecular mass markers. The arrowheads on the right show the protein bands that remained phosphorylated after the KOH treatment. A431 denotes a carcinoma cell line that was included as a positive control. The amount of protein per lane was 100 μg. The results shown are representative of four separate experiments.