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NS4 and VP2 localize with NS7 in infected cells. Permissive microglial cell line BV-2 was infected with NS4-FLAG, VP2-FLAG, or WT virus at an MOI of 1 or mock infected. At 12 hpi, cells were fixed and dual stained for the FLAG tag and NS7. Both NS4 and VP2 colocalized with NS7 in perinuclear foci.
