Table 2.

Validation of insertion profiles by identifying insertions in viruses isolated from 15-nt insertion libraries by limiting dilution

Protein	Nucleotide position	Strain	Amino acid sequencea	Present in profilingb	No. of viruses isolated
NS1-2	218	WT	LAGLPV	No	2
		Mutant	LAGCGRTGLPV
NS4	2523	WT	RPSFYW	Yes	2
		Mutant	RPLRPQPSFYW
NS4	2604	WT	GWYHSE	Yes	1
		Mutant	GWLRPQWYHSE
VPg	2840	WT	ATRRSR	Yes	2
		Mutant	ATRCGRTRRSR
VP1	5073	WT	SDGAAP	Yes	1
		Mutant	SDGCGRNGAAP
VP1	5585	WT	QEESMR	Yes	1
		Mutant	QEDAAAEESMR
VP2	6688	WT	MAGALF	Yes	1
		Mutant	MAGAAAAGALF
VP2	7229	WT	PRDHTP	Yes	1
		Mutant	PRDCGRSDHTP
VP2	7239	WT	TPATQG	Yes	1
		Mutant	TPVRPQPATQG

^aUnderlined residues correspond to the inserted residues; residues in italics indicate a change in residue identity due to insertion within a codon.

^bInsertion detected within selected profiles to within 1 nt.