Table 3.

Sequence and detection of insertions cloned into the WT to validate the profiling

Protein	Nucleotide position	Strain	Amino acid sequencea	Profiling detectionb
				Input	Passage 3
NS1-2	84	WT	SSKASV	4358	31032
		Mutant	SSKVRPHKASV
NS1-2	218c	WT	LAGLPV
		Mutant	LAGCGRTGLPV
NS1-2	382	WT	SHAEDA	407	23352
		Mutant	SHAAAAAHADEA
NS4	2600	WT	DDGWYH	6348	31617
		Mutant	DDGCGRNGWYH
VP2	6686	WT	MAGALF	9181	17900
		Mutant	MAGAAAAGALF
VP2	6688d	WT	MAGALF		618
		Mutant	MAGAAAAGALF
VP2	6722	WT	GLMGII	4736
		Mutant	GLMCGRMMGII
VP2	7229	WT	PRDHTP	308	3399
		Mutant	PRDCGRSDHTP
VP2	7239	WT	TPATQG	283	4262
		Mutant	TPVRPQPATQG

^aUnderlined residues correspond to the inserted residues; residues in italics indicate a change in residue identity due to insertion within a codon.

^bNumbers reflect detection of the insertion to within 1 nt and refer to fluorescence intensity of the corresponding peak.

^cInsertions were not detected in the profiling but were isolated by limiting dilution.

^dInsertion 6688 was not identified in the input profile.