Fig 1.

Strategy for BoHV-4 purification and proteomic analysis by mass spectrometry (MS). (A) MDBK cells were infected with the v.test strain of BoHV-4 at an MOI of 0.05. At 96 hpi, the extracellular medium was harvested. (B and C) BoHV-4 extracellular virions (B) and BoHV-4 capsids (C) were enriched as described in Materials and Methods. (D) The purity of the virions (i and ii) and capsids (iii and iv) preparations was assessed by EM. Bar, 50 nm. Original magnifications, ×30,000 for panels i and iii and ×49,000 for panels ii and iv. (E) Virion-enriched and capsid-tegument preparations were subjected to proteomic analysis by MS using different complementary approaches.