Fig 4.

Analysis of BoHV-4 structural protein glycosylation. Control or deglycosylated proteins of purified BoHV-4 virions were separated by SDS-PAGE. After migration, each sample was divided in 31 serial slices along the lane, and proteins of each slice were identified as described in Materials and Methods. For the two treatments, slices containing gB, gH, or gp180 proteins are shown with color intensity indicating the mean relative abundance (emPAI). For each protein, the predicted molecular mass (MM) is shown (theoretical lane). For the gB protein, the dashed slice indicated the predicted position of gB proteins before potential cleavage by cellular furin. The position of an MM standard is shown on the left.