Table 3.

Cellular proteins detected in BoHV-4 virions and comparison to other herpesviruses^a

Protein description	Accession no.	Relative emPAI (%)b		Gammaherpesvirinae				Alphaherpesvirinae		Beta-
		CTRL	pK	Rhadinoviruses		Maca-	Lymph-	Simpl-	Varicel-	Cyto-
				KSHV	MuHV-4	AlHV-1	EBV	HSV-1	PRV	HCMV
Signaling
14-3-3 protein theta	gi¦5803227	0.25	0.07						+	+
14-3-3 protein gamma	gi¦9507245	0.25	0.07					+		+
Cytoskeleton
Profilin-1	gi¦62751593	1.11	0.14					+
Ezrin	gi¦27806351	0.70	0.03	+
Actin	gi¦4501885	4.30	0.61	+	+	+	+	+		+
KRT18 protein	gi¦148878161	0.47	0.08							+
Cofilin-1	gi¦51592135	2.10	1.11				+	+		+
mRNA binding and processing
Elongation factor 1-alpha 1	gi¦4503471	0.45	0.13				+			+
Membrane organization and trafficking
Annexin 1	gi¦74	0.83	0.31		+	+		+		+
Annexin 2	gi¦27807289	6.40	2.04	+	+	+		+		+
Metabolism
Triosephosphate isomerase	gi¦61888856	0.57	0.26					+		+
Bov mitochondrial F1-ATPase (A)	gi¦1827809	1.10	0.08
Chain B, Bov cytochrome Bc1	gi¦3891849	0.15	0.04
Ion channel
Chloride intracellular channel 1	gi¦62751970	0.57	0.16
Phosphate carrier protein	gi¦27807185	0.17	0.05

^aAbbreviations: Beta-, Betaherpesvirinae; Maca-, macaviruses; Lymph-, lymphocryptoviruses; Simpl-, simplexviruses; Varicel-, varicelloviruses; Cyto-, cytomegaloviruses; Bov, bovine.

^bRelative emPAI values were calculated as the emPAI values calculated for each analysis relative to the abundance of pORF52 taken as 100%. Values > 0.5 are highlighted in boldface. CTRL, values obtained with virion-enriched preparations analyzed by the 1D gel/nano-LC-MS/MS procedure. pK, values obtained with virion-enriched preparations (same as in CTRL) analyzed by the 1D gel/nano-LC-MS/MS procedure after proteinase K treatment. Data indicated for Gammaherpesvirinae, Alphaherpesvirinae, and Betaherpesvirinae are based on previously published studies (2, 3, 5, 8, 17, 20, 23, 30, 42, 45).