Fig 1.

Targeted knockdown of the zebrafish il-10r1 gene using a splice site-blocking morpholino. (A) Sequence targeted by the il-10r1 morpholino. The splice site structure of the zebrafish il-10r1 gene, showing exons (rectangles) and intervening introns (chevrons) with the noncoding regions shaded black, is shown. The il-10r1 splice site-blocking morpholino targets the exon 4/intron 4 splice-site (red line), with the target sequence on the pre-mRNA (gray) and morpholino sequence (red) shown. The exon sequence is in uppercase and the intron sequence in lowercase. (B) Effectiveness of the il-10r1 morpholino in vivo, showing RT-PCR of RNA extracted from 56-hpf zebrafish embryos injected with il-10r1 splice site-blocking or control morpholinos, as indicated, including samples not treated with reverse transcriptase (RT-ve). The black arrowhead indicates the wild-type transcript in the standard control embryos that is robustly ablated in il-10r1 morpholino-injected embryos, with alternate splicing products indicated with red arrowheads.