Fig 4.

Expression of the khvIL-10 gene (A, C, and E) and carp IL-10 gene (B, D, and F) in carp tissues during the acute, persistent, and reactivation phases of KHV infection. Transcripts were quantified using a TaqMan qRT-PCR assay based on comparative threshold cycle (ΔΔCT) and normalized against expression of the 18S rRNA gene. Data are presented as the means ± standard errors for four individual fish per group. Asterisks indicate significant differences (P < 0.05) in expression between acute/reactivation and persistent phases of KHV infection. Note the difference in scale for KHV versus carp genes.