Fig 6.

Functional conservation between khvIL-10 and Danio rerio IL-10. (A to D) Both khvIL-10 and drIL-10 increase embryonic leukocyte numbers. Embryos were injected with standard control morpholino (StdCon) alone (A) or coinjected with mRNA encoding either khvIL-10 (B) or Danio rerio IL-10 (drIL-10) (C) and subjected to WISH using the pan-leukocytic marker (lyz). The number of individual blue-stained lyz⁺ cells was quantified by manual counting (D), showing the mean (dashed red line), 95% confidence interval (red lines), and level of statistical significance (P < 0.01, *). (E to H) Both khvIL-10 and drIL-10 require IL-10R1. Embryos were injected with il-10r1SSMo alone (E) or coinjected with either khvIL-10 (F) or dril-10 (G), analyzed by WISH using lyz, and quantified (H), as described above.