Table 1.

Binding affinities of selected prosurvival Bcl-2 proteins for BH3 ligands

BH3 ligand	Binding affinity (IC50a in nM)
	SPPV14	M11L	BHRF1	Bcl-2	Bcl-w	Bcl-xL	Mcl-1	A1
Bad	>2,000	>1,000	>2,000	16	30	5.3	>100,000	15,000
Bid	341 ± 16	100	42	6,800	40	82	2,100	1
Bik	>2,000	>1,000	>2,000	850	12	43	1,700	58
Bim	26 ± 4	5	<50	2.6	4.3	4.6	2.4	1
Bmf	67 ± 6	100	>2,000	3	9.8	9.7	1,100	180
Hrk	63 ± 6	>1,000	>1,000	320	49	3.7	370	46
Noxa	>2,000	>1,000	>2,000	>100,000	>100,000	>100,000	24	20
Puma	65 ± 1	>1,000	<50	3.3	5.1	6.3	5	1
Bak	46 ± 3	50	NA	>1,000	500	50	10	NA
Bax	32 ± 5	75	NA	100	58	130	12	NA
Bim2A	>1,000	NA	NA	>10,000	>10,000	>10,000	19	520
ABT-737	>1,000	NA	>20,000	3.5	9.5	5.7	>2,000	>2,000

^aIC₅₀s were determined by solution competition assays using the Biacore optical biosensor (9) and represent means ± SD from 3 independent experiments. NA, not available. Apart from SPPV14, data were taken from references 9, 22, 40* to 42*, and 67*.