Fig 8.

Estimation of RT content of virions. (A) DF1 cells were infected with pRCASwt, pRCASIBV2, or pRCAS25IBV2, supernatant virus was harvested by centrifugation, and serial dilutions were subjected to Western blotting with the anti-p27 antibody (left) and to RT activity assays (right). The graphs show quantification of p27 and RT levels for neat virus and three dilutions. (B) The RT/p27 ratio at each dilution was calculated for each virus (left) and is shown normalized to the ratio for pRCASwt (right).