Table 1.
Frameshift efficiencies measured in RRL for pMV-RCAS and mutant derivatives at 30°C or 39°Ca
| Construct | Assay details | Frameshift efficiency (%) |
|---|---|---|
| RCAS (WT) | Wild-type RCAS frameshift signal present | 4.0 |
| 9 | Destabilized PK through modification of PK1 | 0.2 |
| 11 | Destabilized PK through modification of PK2 | 1.6 |
| 12 | Destabilized PK through modification of PK4 | 1.6 |
| 20 | Inserted stop codon in pol frame (Gag only) | 0.0 |
| 22 | Modulation of stem 1 (3-bp extension) | 7.6 |
| 23 | Modulation of stem 1 (1-bp extension) | 6.0 |
| 25 | Modulation of stem 1 (4-bp extension) | 9.4 |
| ALV | Wild-type ALV frameshift signal present | 7.8 |
| ALV-C | ALV signal with U2502 changed to C2502 | 3.5 |
| RSV | Wild-type RSV frameshift signal present | 3.4 |
| S2 | Destabilized stem 2 (part of ISE) | 3.6 |
| S3 | Destabilized stem 3 (part of ISE) | 1.1 |
| 12-IBV | SS changed to UUUAAAC in context of RCAS-12 | 4.2 |
| 25-IBV | SS changed to UUUAAAC in context of RCAS-25 | 15.0 |
| WT | Wild-type RCAS frameshift signal present | 4.4 (4.6) |
| 12 | Destabilized PK through modification of PK4 | 1.9 (1.4) |
| 25 | Modulation of stem 1 (4-bp extension) | 12.8 (7.8) |
| SRV | SS changed to AAAUUUU | 8.3 (7.5) |
| 12-SRV | SS changed to AAAUUUU in context of RCAS-12 | 4.3 (3.0) |
| 25-SRV | SS changed to AAAUUUU in context of RCAS-25 | 17.4 (13.5) |
| IBV2 | AAC inserted immediately downstream of natural SS to generate IBV SS | 6.5 (6.1) |
| 12-IBV2 | AAC inserted immediately downstream of natural SS to generate IBV SS in context of RCAS-12 | 4.4 (2.0) |
| 25-IBV2 | AAC inserted immediately downstream of natural SS to generate IBV SS in context of RCAS-25 | 18.2 (16.8) |
a
Values in parentheses are for translations that were carried out at 39°C. SS, slippery sequence.