Fig 5.

Stable expression of U20 inhibits TNF-α-induced apoptosis. (A) PCR with the U20 ORF spanning primers on cDNA from HCT116 wt or HCT116-U20S cells. M, base pair markers. (B) Real-time PCR with primers against U20 or β-actin on mRNA extracted from HCT116 wt, U20-S, and HHV-6B-infected HCT116 wt (0, 24, and 48 h p.i.) cells. (C) HCT116 wt and U20-S cells were left untreated or treated with TNF-α (25 ng/ml) and CHX (10 μM) for 4 h, followed by Western blotting with antibodies against PARP or GAPDH (loading control). (D) Quantification of cleaved PARP from panel C. (E) HCT116 wt and U20-S cells were left untreated or treated with CPT for 18 h, followed by Western blot analyses with antibodies against PARP or GAPDH (loading control). (F) Quantification of cleaved PARP from panel E.