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. 2012 Nov;86(21):11608–11615. doi: 10.1128/JVI.01260-12

Table 1.

List and characterization of Alix-Bro1 mutants tested in this studya

Provisional name Alix Bro1 domain mutants NC binding CHMP4B binding SAS value (Å2)
Q8 Q8/K11 − + 130, 150
E14/D16/K19 ND ND 131, 82, 128
K23/F24/Q26/Q27 + + 128, 65, 102, 145
E35/R41 + + 98, 172
E44/E45 + + 99, 60
K2R K48/R51/R56 − + 131, 90, 125
Q8/K2R Q8/K11/K48/R51/R56 − + 130, 150, 131, 90, 125
Q8/K2RK Q8/K11/K48/R51/R56/K60 − + 130, 150, 131, 90, 125, 104
D59/E62 + + 130, 130
F84/S85/E86 + + 141, 97, 62
D100/K101 + + 93, 148
L104 + + 133
F105/G106/G107 + + 193, 91, 59
L104/F105/G106/G107 + + 133,193, 91, 59
F105/G106/G107/K110 + + 193, 91, 59, 124
K110 + + 124
E137/D141/N142/D143/E144 ND ND 108,83, 71, 56, 109
D141/D143/E144 + + 83, 56, 109
K164/E165 + + 72, 134
S172/R173/E174 + + 92,137,174
a

Residues belonging to the Phe105 loop (36) are shaded gray. ND, not determined because of low or no expression. Data for mutants in boldface are shown in Fig. 1, 2, and 3. Plus and minus symbols indicate positive and negative (absence) binding, respectively. SAS, solvent accessible surface.