Fig 1.

Selection of OMP microarray candidates. Three hundred and 61 genes were included in the leptospiral OMP microarray. All annotated lipoproteins (n = 177), OMPs (n = 97), and leucine-rich-repeat proteins (n = 13) were included. Additional transmembrane OMPs were identified by the following criteria: (i) the presence of a signal peptide with signal peptidase cleavage site by http://www.cbs.dtu.dk/services/SignalP and (http://www.cbs.dtu.dk/services/LipoP/, (ii) the absence of more than three inner membrane-spanning α-helices by TMHMM (www.cbs.dtu.dk/services/TMHMM), and (iii) the prediction of at least six membrane-spanning β-strands by either PRED-TMBB (http://biophysics.biol.uoa.gr/PRED-TMBB/) or TMBETA-NET (http://psfs.cbrc.jp/tmbeta-net/).