Fig 3.

Leptospiral OMP microarray proteins with the best fibronectin-binding activity. The leptospiral OMP microarray was probed with 10 μg of fibronectin/ml. The MFIs were calculated using ImageJ, version 1.44 software (http://rsb.info.nih.gov/ij/). The annotation and MFI values of 15 leptospiral proteins that exhibited significant binding based on an arbitrary threshold of 10 MFIs are shown. LIC11755-s1 and LIC11028-s4 denote fragments of these proteins that were cloned as 2-kb segments due to large sizes of these ORFs (>3,000 bp).