Fig 6.
Expression of MFn proteins in cultivated L. interrogans. Lanes contain 108 leptospires or 0.5 μg of recombinant proteins separated by gel electrophoresis, blotted onto PVDF membrane, and probed with rabbit immune sera specified below each blot. rMFn1, rMFn7, rMFn8, and rMFn9 denote the corresponding recombinant proteins. Lane WC contains L. interrogans serovar Copenhageni strain Fiocruz L1-130 whole-cell lysate. The asterisk indicates a 3-fold increase in the amount of whole-cell lysate (3 × 108) loaded into wells. The identities of individual proteins are indicated on the right, and the positions of the molecular mass standards (in kilodaltons) are indicated on the left.