Table 1.
Primers for amplification of genes for expression in E. coli
| Oligonucleotide | Sequence (5′–3′)a | Gene |
|---|---|---|
| Z1_11612F | ATGTACATATGGAAGCGGGTGGACTTA | mfn1 |
| Z1_11612R | GTAAACTCGAGTTCCAATTCCCAGGAT | mfn1 |
| Z2_10714F | GAAGTTCATATGCAATCAGAAGAAACAAA | mfn2 |
| Z2_10714R | AACAACTCGAGAAGTTCAAACGTGG | mfn2 |
| Z6_11051F | GAAACATATGAAACACTACCTAACCT | mfn6 |
| Z6_11051R | GTAGAACTCGAGAGAAAGATAAAGTACT | mfn6 |
| Z7_11436F | AAGTTACATATGAAATACTTGACTGAAGA | mfn7 |
| Z7_11436R | AAAGACTCGAGTTCCACATAAATTT | mfn7 |
| Z8_10258F | CACTTCATATGAATACTTTTTTAAAGACG | mfn8 |
| Z8_10258R | CATATGTCGACAAGTGAAAGATAAAAAT | mfn8 |
| Z9_10537F | GTCTTCATATGGAGAATAAAAATTCTTCC | mfn9 |
| Z9_10537R | AAAAAACTCGAGTTTACTTTTTTTCAAAATC | mfn9 |
a
Restriction sites (CATATG for NdeI, CTCGAG for XhoI) are underlined.