Table 2.
Oligonucleotides for cloning of genes in L. biflexa
| Oligonucleotide | Sequence (5′–3′)a | Geneb |
|---|---|---|
| flaB1p(Kp)-1F | CTCGCTGGTACCGATCGAACCTAAGATTAGCTCA | PromflaB1 |
| flaB1p(Xh)-2R | GTCCTCGAGTTCCATATGTTTCCTCCTTGAAACTGATC | PromflaB1 |
| Z1_11612fF | ACGTCATATGTTTCAAAACGTTTTCAA | mfn1 |
| Z1_11612SR | AGTACTCGAGTTATTCCAATTCCCAGG | mfn1 |
| Z3_13198fF | ATGTCATATGAAAACAAAACGAAATC | mfn3 |
| Z3_13198SR | GTAAAACTCGAGCTAACGATAAATTAGATC | mfn3 |
| Z4_12631F | AGAGACATATGATAAACAAAATAACAAAACC | mfn4 |
| Z4_12631SR | AGGACTCGAGTTAGCGATAAATAAGAT | mfn4 |
| Z7_11436SR | ATAAACTCGAGTTATTCCACATAAATT | mfn7 |
| Z9_10537fF | GAAAACATATGCATACCCTGCT | mfn9 |
| Z9_10537SR | ATAAACTCGAGTTATTTACTTTTTTTCAAAA | mfn9 |
| Z12_11028s4F | GCTCATATGTATCGCTCTTTTTTAA | mfn12 |
| Z12_11028s4R | TTATCCTCGAGCTATTCTTCTAATATAATACT | mfn12 |
| pRAT575_MPForv | CTAAATCGGAACCCTAAAGG | pRAT575 inserts |
| pRAT575_MPRev | CCACAATCAGACAATGACC | pRAT575 inserts |
a
Restriction sites (GGTACC for KpnI, CATATG for NdeI, and CTCGAG for XhoI) are underlined.
b
The forward primer Z7_11436F (Table 1) was used for cloning in both E. coli and L. biflexa.