Table 2.
Oligonucleotide | Sequence (5′–3′)a | Geneb |
---|---|---|
flaB1p(Kp)-1F | CTCGCTGGTACCGATCGAACCTAAGATTAGCTCA | PromflaB1 |
flaB1p(Xh)-2R | GTCCTCGAGTTCCATATGTTTCCTCCTTGAAACTGATC | PromflaB1 |
Z1_11612fF | ACGTCATATGTTTCAAAACGTTTTCAA | mfn1 |
Z1_11612SR | AGTACTCGAGTTATTCCAATTCCCAGG | mfn1 |
Z3_13198fF | ATGTCATATGAAAACAAAACGAAATC | mfn3 |
Z3_13198SR | GTAAAACTCGAGCTAACGATAAATTAGATC | mfn3 |
Z4_12631F | AGAGACATATGATAAACAAAATAACAAAACC | mfn4 |
Z4_12631SR | AGGACTCGAGTTAGCGATAAATAAGAT | mfn4 |
Z7_11436SR | ATAAACTCGAGTTATTCCACATAAATT | mfn7 |
Z9_10537fF | GAAAACATATGCATACCCTGCT | mfn9 |
Z9_10537SR | ATAAACTCGAGTTATTTACTTTTTTTCAAAA | mfn9 |
Z12_11028s4F | GCTCATATGTATCGCTCTTTTTTAA | mfn12 |
Z12_11028s4R | TTATCCTCGAGCTATTCTTCTAATATAATACT | mfn12 |
pRAT575_MPForv | CTAAATCGGAACCCTAAAGG | pRAT575 inserts |
pRAT575_MPRev | CCACAATCAGACAATGACC | pRAT575 inserts |
Restriction sites (GGTACC for KpnI, CATATG for NdeI, and CTCGAG for XhoI) are underlined.
The forward primer Z7_11436F (Table 1) was used for cloning in both E. coli and L. biflexa.