Table 3.

Summary of OMP microarray-identified fibronectin-binding proteins

MFn protein	Purified rMFna	Antiserum produced	Expression in cultivated L. interrogans (size [kDa])	Surface proteolysis	Solid-phase Fn bindingc	Overexpression in L. biflexa transformants	Presence of homologs in L. biflexa	Acquisition of Fn by L. biflexa transformants
MFn1	D	Whole protein	Yes (48)	Yes	3	Yes	No	Yes
MFn2	D	NAd	NDb	ND	1	NA	Yes	NA
MFn3 (Sph3)	NA	Synthetic peptide	Yes (90)	ND	ND	No	No	No
MFn4 (Sph2)	NA	N-terminal fragment (amino acids 27 to 190)f	Yes (76)	ND	ND	Yes	No	Yes
MFn6	D	NA	ND	ND	4	NA	No	NA
MFn7	D, N	Whole protein	Yes (80)	Yes	2e	Yes	Yes	Yes
MFn8	D	Whole protein	No (66)	ND	2	NA	No	NA
MFn9	D	Whole protein	Yes (75)	Yes	5	No	Yes	No
MFn12	NA	NA	ND	ND	ND	ND	Yes	No

^aD or N, purified under denaturing or native conditions, respectively.

^bND, not determined.

^cOrdered dose-dependent strength of binding: 1 = strongest.

^dNA, not applicable.

^eStrongest binding at lower amounts, receptor-like characteristics; only native protein binds.

^fData from reference 62.