Table 1.
NMR structure parameters
| Parameter | Value |
|---|---|
| NMR distance and dihedral constraints | |
| Distance constraints | |
| Total NOE | 1,188 |
| Intraresidue | 240 |
| Interresidue | |
| Sequential (¦i − j¦ = 1) | 358 |
| Medium-range (¦1 < i − j¦ < 5) | 210 |
| Long-range (¦i − j¦ ≥ 5) | 380 |
| Hydrogen bonds | 0 |
| Total dihedral angle restraints | |
| ϕ | 69 |
| ψ | 66 |
| X1 | 17 |
| Structure statistics | |
| Violations (mean and SD) | |
| Max.a distance constraint violation (Å) | 0.3 |
| Max. dihedral angle violation (°) | 5.0 |
| Deviations from idealized geometry | |
| Bond lengths (Å) | 0.0117 ± 0.0001 |
| Bond angles (°) | 1.2167 ± 0.0072 |
| Impropers (°) | 1.3773 ± 0.0262 |
| Ramachandran plot (%) | |
| Most-favored region | 87.1 |
| Additionally allowed region | 12.1 |
| Generously allowed region | 0.8 |
| Disallowed region | 0.0 |
| Avg pairwise RMS deviationb (Å) (residues 143–229) | |
| Backbone | 0.84 ± 0.11 |
| Heavy | 1.56 ± 0.13 |
a
Max., maximal.
b
Pairwise RMS deviation was calculated among 15 refined structures.