Table 1.
Strains and plasmids used in this study
| Strain | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | F− ϕ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Laboratory stock |
| BW25113/pIJ790 | K-12 derivative; ΔaraBAD ΔrhaBAD/λ-Red (gam bet exo) cat araC rep101(Ts) | 19 |
| ET12567/pUZ8002 | dam-13::Tn9 dcm cat tet hsdM hsdR zjj-201::Tn10/tra neo RP4 | 36 |
| S. coelicolor | ||
| M145 | SCP1− SCP2− | 2 |
| FM145 | Derivative of M145 with reduced autofluorescence | 55 |
| TP5 | M145 ΔtatB | 51 |
| TP6 | TP5 harboring pTDW134 | This work |
| TP7 | TP5 harboring pTDW135 | This work |
| BRO1 | M145 tatA::tatA-egfp aac3(IV)-oriT | This work |
| BRO2 | M145 tatC::tatC-egfp aac3(IV)-oriT | This work |
| BRO3 | FM145 tatA::tatA-egfp aac3(IV)-oriT | This work |
| BRO4 | FM145 tatC::tatC-egfp aac3(IV)-oriT | This work |
| BRO5 | BRO3 harboring pTDW136 | This work |
| BRO6 | BRO4 harboring pTDW136 | This work |
| BRO7 | BRO3 harboring pTDW137 | This work |
| BRO8 | FM145 harboring pTDW135 | This work |
| Plasmids | ||
| pTDW134 | pIJ8660 tatBp-tatBstop-egfp | This work |
| pTDW135 | pIJ8660 tatBp-tatB-egfp | This work |
| pTDW136 | pIJ6902 tatB-mcherry | This work |
| pTDW137 | pIJ6902 tatC-mcherry | This work |