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Spot plate assays of diffocin 4 and the hybrid diffocin 4-16 on target strains 19145, 19099, 19137, and 19135. Diffocin 4 was produced in B. subtilis BDG45, and the hybrid diffocin 4-16 was produced in BDG55. The diffocins were purified, and serial 5-fold dilutions of diffocins were spotted onto lawns of target bacteria, with diffocin 4 on the left and diffocin 4-16 on the right of each lawn. The plates were incubated overnight at 37°C before imaging.
