Table 2.
Effect on prespore-specific transcription of different perturbations of chromosome translocation and replication
| Strain | Perturbationa | σ tested | Promoterb | No. with GFP fluorescence/total at stage |
|||
|---|---|---|---|---|---|---|---|
| IIi | IIii | IIiii | III | ||||
| SL14360 | None | σG | PsspA | 0/263 | 0/87 | 0/59 | 432/439 |
| SL14361 | 0.9-Mb insert | σG | PsspA | 0/169 | 0/46 | 0/60 | 365/372 |
| SL14378 | 2.3-Mb insert | σG | PsspA | 0/564 | 0/212 | 0/153 | 505/523 |
| SL14539 | 2.3-Mb insert | σF | PspoIIQ(ter) | 0/445 | 0/227 | 14/218 | 261/261 |
| SL14856 | 2.3-Mb insert | σF | PspoIIQ(ori) | 15/196 | 124/147 | 261/262 | 806/806 |
| SL15673 | 2.3-Mb insert | σF/σG | PsigGc | 49/308 | 196/273 | 306/325 | 1,026/1,027 |
| SL15103 | Ter at 145° | σG | PsspA | 0/291 | 0/207 | 0/190 | 270/353 |
| SL14469 | Slow translocase | σG | PsspA | 0/94 | 0/37 | 0/36 | 177/178 |
a
Insertion of Synechocystis DNA, termination of replication (Ter) at 145°, or slow SpoIIIE (D584A) DNA translocase.
b
Promoter driving expression of gfp. ter and ori, promoter located near the terminus and origin.
c
Promoter of the structural gene for σG.