Table 1.
Summary of disruption of ribosomal protein genes
| Gene | Protein | Transcriptional unit | Replacementa | Gene disruption in B. subtilis | Essentiality in E. colib |
|---|---|---|---|---|---|
| rplA | L1 | rplK-rplA | catΔpt1 | Yes | Nonessential |
| rplB | L2 | S10-spc-α | catΔpt1 | No | Essential |
| rplC | L3 | S10-spc-α | catΔpt1 | No | Essential |
| rplD | L4 | S10-spc-α | catΔpt1 | No | Essential |
| rplE | L5 | S10-spc-α | catΔpt1 | No | Essential |
| rplF | L6 | S10-spc-α | catΔpt1 | No | Essential |
| rplI | L9 | yybS-yybT-rplI | cat | Yes | Nonessential |
| rplJ | L10 | rplJ-rplL | cat | No | Essential |
| rplK | L11 | rplK-rplA | Yesc | Nonessential | |
| rplL | L7/L12 | rplJ-rplL | cat | No | Essential |
| rplM | L13 | rplM-rpsI | catΔpt1 | No | Essential |
| rplN | L14 | S10-spc-α | catΔpt1 | No | Essential |
| rplO | L15 | S10-spc-α | catΔpt1 | Yes | Nonessential |
| rplP | L16 | S10-spc-α | catΔpt1 | No | Essential |
| rplQ | L17 | S10-spc-α | cat | No | Essential |
| rplR | L18 | S10-spc-α | catΔpt1 | No | Essential |
| rplS | L19 | rplS | cat | No | Essential |
| rplT | L20 | infC-rpmI-rplT | cat | No | Essential |
| rplU | L21 | rplU-ysxB-rpmA | cat | No | Nonessential |
| rplV | L22 | S10-spc-α | catΔpt1 | Yes | Essential |
| rplW | L23 | S10-spc-α | catΔpt1 | Yes | Essential |
| rplX | L24 | S10-spc-α | catΔpt1 | No | Nonessential |
| ctc | L25 homologue | ctc | Yesc | Nonessential | |
| rpmA | L27 | rplU-ysxB-rpmA | cat | No | Nonessential |
| rpmB | L28 | rpmB | cat | Yes | Essential |
| rpmC | L29 | S10-spc-α | catΔpt1 | Yes | Nonessential |
| rpmD | L30 | S10-spc-α | catΔpt1 | No | Nonessential |
| rpmE | L31 | rpmE | cat | Yesc | Nonessential |
| ytiA | L31 homologue | ytiA | cat | Yesc | — |
| rpmF | L32 | rpmF | cat | Yes | Nonessential |
| rpmGA | L33.1 | rpmGA | cat | Yesc | Nonessential |
| rpmGB | L33.2 | rpmGB | cat | Yesc | — |
| rpmH | L34 | rpmH | cat | Yes | Nonessential |
| rpmI | L35 | infC-rpmI-rplT | catΔpt1 | Yes | Nonessential |
| rpmJ | L36 | S10-spc-α | catΔpt1 | Yes | Nonessential |
| ypfD | S1 homologue | ypfD-cmk | Yesc | Essential | |
| rpsB | S2 | rpsB | cat | No | Essential |
| rpsC | S3 | S10-spc-α | catΔpt1 | No | Essential |
| rpsD | S4 | rpsD | cat | No | Essential |
| rpsE | S5 | S10-spc-α | catΔpt1 | No | Essential |
| rpsF | S6 | rpsF-ssb-rpsR | catΔpt1 | Yes | Nonessential |
| rpsG | S7 | rpsL-rpsG-fus | catΔpt1 | No | Essential |
| rpsH | S8 | S10-spc-α | catΔpt1 | No | Essential |
| rpsI | S9 | rplM-rpsI | cat | No | Nonessential |
| rpsJ | S10 | S10-spc-α | catΔpt1 | No | Essential |
| rpsK | S11 | S10-spc-α | catΔpt1 | No | Essential |
| rpsL | S12 | rpsL-rpsG-fus | catΔpt1 | No | Essential |
| rpsM | S13 | S10-spc-α | catΔpt1 | No | Essential |
| rpsN | S14 | S10-spc-α | catΔpt1 | No | Essential |
| yhzA | S14 homologue | yhzA | cat | Yesc | — |
| rpsO | S15 | rpsO | cat | No | Nonessential |
| rpsP | S16 | rpsP | catΔpt1 | No | Essential |
| rpsQ | S17 | S10-spc-α | catΔpt1 | No | Nonessential |
| rpsR | S18 | rpsF-ssb-rpsR | cat | No | Essential |
| rpsS | S19 | S10-spc-α | catΔpt1 | No | Essential |
| rpsT | S20 | rpsT | cat | Yes | Nonessential |
| rpsU | S21 | rpsU-yqeT | catΔpt1 | Yes | Nonessential |