Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| D. dadantii 3937 derivatives | ||
| A350 | rafR ganB | 17 |
| A5275 | rafR ganB bamE::Kanr | This study |
| A5276 | rafR ganB bamC::Kanr | This study |
| A5281 | rafR ganB bamB::Kanr | This study |
| A5476 | rafR ganB degP::Kanr | This study |
| A5477 | rafR ganB skp::uidA Kanr | This study |
| A5478 | rafR ganB surA::Kanr | This study |
| Escherichia coli | ||
| NM522 | F′ proAB lacIq Δ(lacZ)M15 Δ(lac-proAB) thi hsd-5 | Stratagene |
| SR1458 | F− araD139 Δ(argF-lac)U169 ϕ(PdegP-lacZ) | 36 |
| Plasmids | ||
| pGEM-T | Ampr; allows the AT cloning of a PCR product | Promega |
| pBluescript | pUC ori Ampr | Stratagene |
| pACT3 | pACYC ori Cmr | 23 |
| pL247 | Ampr uidA::Kanr cassette | Laboratory collection |
| pHP45ΩKan | pMB1 ori Ampr Kanr cassette | 7 |
| pGEX-6P3 | pBR322 ori Ampr, codes for GST | GE Healthcare |
| pGEM-T surA | Contains the DNA fragment amplified with SurA+ and SurA− | This study |
| pGEM-T surA::Kanr | pHP45ΩKan Kanr cassette in the HindIII site of pGEM-T surA | This study |
| pGEM-T skp | Contains the DNA fragment amplified with Skp+ and Skp− | This study |
| pGEM-T skp::Kanr | pL247 uidA::Kanr cassette in the PstI site of pGEM-T skpΔPstI | This study |
| pGEM-T degP | Contains the DNA fragment amplified with DegP+ and DegP− | This study |
| pGEM-T degP::Kanr | pHP45ΩKan Kanr cassette in the MunI site of pGEM-T degP | This study |
| pGEM-T bamB | Contains the DNA fragment amplified with BamB+ and BamB− | This study |
| pGEM-T bamB::kanr | pHP45ΩKan Kanr cassette in the BclI site of pGEM-T bamB | This study |
| pGEM-T bamC | Contains the DNA fragment amplified with BamC+ and BamC− | This study |
| pGEM-T bamC::kanr | pHP45ΩKan Kanr cassette in the BclI site of pGEM-T bamC | This study |
| pGEM-T bamE | Contains the DNA fragment amplified with BamE+ and BamE− | This study |
| pGEM-T bamE::kanr | pHP45ΩKan Kanr cassette between BamHI sites of pGEM-T bamE | This study |
| pGEM-T pnlH | Contains the DNA fragment amplified with PnlHHis+ and PnlHHis− | This study |
| pBSpnlH | Insertion of pGEM-T pnlH ApaI SacI DNA fragment containing pnlH CDS in pBS | This study |
| pPnlH | pACT3 containing the gene of PnlH with a C-terminal His tag between SacI and KpnI sites | This study |
| PnlH-PemA | pBAD18 containing a fusion of the PnlH signal sequence with the mature form of PemA | 8 |
| pPnlHssPemA | pACT3 containing a fusion of the PnlH signal sequence with the mature form of PemA | This study |
| pD16A | pPnlH containing a D16A substitution in PnlH | This study |
| pL30SL31T | pPnlH containing L30SL31T substitutions in PnlH | This study |
| pP32L | pPnlH containing a P32L substitution in PnlH | This study |
| pF34A | pPnlH containing a F34A substitution in PnlH | This study |
| pP35A | pPnlH containing a P35A substitution in PnlH | This study |
| pP32LP35A | pPnlH containing P32LP35A substitutions in PnlH | This study |
| pY36A | pPnlH containing a Y36A substitution in PnlH | This study |
| pQ41A | pPnlH containing a Q41A substitution in PnlH | This study |
| pPnlHΔ28-41 | pPnlH containing a deletion of nucleotides 81 to 123 | This study |
| pSurA | pGEM-T containing SurA with a C-terminal Strep tag under the control of the lac promoter | This study |
| pXss20 | pGEX-6P3 containing of a fusion of GST with the first 41 amino acids of PnlH | This study |