Table 2.
Growth inhibition of D. dadantii mutants for the OMP targeting pathwaya
| Strain | Characteristic or mutated gene | Inhibition zone diam (mm) |
||
|---|---|---|---|---|
| SDS (10%) | EDTA (0.5 M) | Rifampin (20 mg/ml) | ||
| A350 | Wild type | 11 ± 0 | 19 ± 2 | 16 ± 1 |
| A5275 | bamE | 14 ± 1 | 29 ± 1 | 21 ± 1 |
| A5276 | bamC | 13 ± 1 | 20 ± 1 | 17 ± 1 |
| A5281 | bamB | 19 ± 1 | 32 ± 1 | 22 ± 0 |
| A5476 | degP | 12 ± 1 | 24 ± 2 | 16 ± 1 |
| A5477 | skp | 18 ± 1 | 30 ± 1 | 25 ± 1 |
| A5478 | surA | 17 ± 0 | 31 ± 1 | 22 ± 1 |
a
Sensitivity to SDS (10%), EDTA (0.5 M), and rifampin (20 mg/ml) was determined by measuring the growth inhibition zone on LB agar plates topped with filter paper disks containing these compounds, as described in Materials and Methods. The values indicated are the means of 3 experiments.