Fig 1.

(A) Structure of 35B2. (B) Confirmation of antiviral activity of 35B2. HEL cells were plated in wells of 24-well plates, infected with 2,000 PFU of cell-free VZV (V-Oka), cultured in the presence of DMSO (closed circles), 20 μM 35B2 (open circles), and 36 μM (8 μg/ml) ACV (closed squares), and then harvested at the indicated time points. VZV copy numbers per cell were obtained by real-time PCR assays for the VZV IE62 gene and the human albumin gene. The relative amounts of viral DNA under each of the conditions are shown by using the copy numbers per cell obtained from the infected cells treated with DMSO at 70 h after infection as a 100% control. The experiments were done in triplicate, and the means of the relative amounts and the standard deviations are shown in the graph. (C) HEL cells were plated in the wells of 12-well plates, infected with 6,000 PFU of cell-free VZV (V-Oka), and cultured in the presence of DMSO, 20 μM 35B2, and 20 μg/ml ACV. At 18 h after infection, nuclei were prepared from the infected cells. VZV copy numbers per cell were obtained as described above, and the relative amounts of viral DNA are shown by using the copy numbers per cell obtained from the infected cells treated with DMSO as a 100% control.