Fig 5.

No effect of 35B2 on the scaffold protein-mediated translocation of the VZV MCP. GPL cells were plated in media containing DMSO (left) or 20 μM 35B2 (right), transfected with phCMV-ORF40 along with pCMV-ORF33.5F expressing FLAG-tagged ORF33.5 (ORF40+ORF33.5) or with an empty vector (ORF40+vector), cultured for 2 days, and then fixed with acetone. FLAG-tagged scaffold protein (ORF33.5F) and MCP (ORF40) were visualized by IFA using the anti-FLAG tag monoclonal antibody and the rabbit anti-GST-ORF40 polyclonal antibody as primary antibodies, Alexa Fluor 594-conjugated anti-mouse IgG Fab′ and FLAG-conjugated anti-rabbit IgG Fab′ as secondary antibodies, and DAPI for counterstaining. Alexa Fluor 594, FITC, and DAPI signals are shown in red, green, and blue, respectively.