Fig 7.

Effect of 35B2 on localization of the VZV MCP in infected cells. (A and B) GPL cells were infected with cell-free virus (V-Oka), cultured in the presence of DMSO (A) or 20 μM 35B2 (B) and fixed 26 h after infection. (C through H) GPL cells were infected with the cell-associated virus V-Oka (C and D) and 35B2-resistant clones R1 (E and F) and G18 (G and H), cultured in the presence of DMSO (C, E, and G) or 20 μM 35B2 (D, F, and H), and fixed 44 h after infection. The cells were reacted with rabbit anti-GST-ORF40 and then with FITC-conjugated anti-rabbit IgG and counterstained with DAPI. The top panels show only FITC signals (green), and the bottom panels show both FITC (green) and DAPI (blue) signals.