Fig 2.

Intracerebral inoculation of BJCA08/CA16 results in dose-related disease and mortality. One-day-old ICR mice (n = 8 to 10 per group) were intracerebrally inoculated with increasing doses of BJCA08 (0.3, 2.4, 1.9 × 10, 3.0 × 10², and 1.9 × 10⁴ PFU/mouse). Control animals were given medium instead of a virus. Mortality and clinical disease were monitored and recorded daily after infection. One representative from two independent experiments is shown. The LD₅₀ was calculated as 1.2 PFU/mouse, and 3.0 × 10² PFU/mouse (260 LD₅₀) was chosen as the challenge dose.