Fig 3.

Histological examination of infected mice after intracerebral challenge with lethal doses of BJCA08/CA16. One-day-old ICR mice were intracerebrally inoculated with medium (mock control) or BJCA08 (260 LD₅₀). (a) A representative picture of wasting caused by BJCA08 at day 8 of postinfection is shown (arrow). (b) A representative picture of right-hind-limb paralysis caused by BJCA08 at day 8 of postinfection is shown (arrow). Mice on the right side of panels a and b labeled in yellow were naive age-matched controls. Representative sections are shown. (c) Infected mice (grades 4 to 5) exhibited severe necrotizing myositis in hind-limb skeletal muscles (arrow). (d) Severe necrotizing myositis in skeletal muscles near the spine (arrow) was also detected in infected mice (grades 4 to 5). (e) A moribund mouse due to BJCA08 exhibited focal myocarditis (arrow). (f) In contrast, no histological change was observed in the heart of the mock-infected control mice. No histological change in the brain was observed in the brain of the infected mice (grades 3 to 5) (g) or the mock control mice (h). Samples in panels c to h were stained with hematoxylin and eosin stain; magnification, ×400.