Table 2.
HSV-1(F) recombinant viruses used
| Virus | Mutation(s) | Resulting coding change(s) | Source |
|---|---|---|---|
| WT | None | None | 7 |
| UL49− | Deletion of HSV-1 bp 105486 to 106391 | Deletion of VP22 (UL49 ORF deletion) | 7 |
| vhsFS | Guanine nucleotide insertion at HSV-1 bp 91789 | Frameshift at vhs residue 284 | This work |
| UL49−/vhsFS | Deletion of HSV-1 bp 105486 to 106391 and guanine nucleotide insertion at HSV-1 bp 91789 | Deletion of VP22 (UL49 ORF deletion) and frameshift at vhs residue 284 | This work |
| vhsD213N | C-to-T nucleotide change at HSV-1 bp 92001 | Aspartic acid (D)-to-asparagine (N) change at vhs residue 213 | This work |
| UL49−/vhsD213N | Deletion of HSV-1 bp 105486 to 106391 and C-to-T nucleotide change at HSV-1 bp 92001 | Deletion of VP22 (UL49 ORF deletion) and aspartate (D)-to-asparagine (N) change at vhs residue 213 | This work |