Fig 2.

ZAP inhibits M2 expression. (A) Schematic representation of M2 gene segments. gDNA, genomic DNA. (B) The CDS alone or CDS-3′ UTR of M2 was cloned into a protein expression vector, followed by transfection into 293TRex-rZAP cells. At 6 h posttransfection, cells were treated with tetracycline to induce ZAP expression. At 54 h posttransfection, cells were lysed and the expression of Flag-tagged M2 was detected by Western blotting. (C) The 3′ UTR of M2 was cloned into pGL3-Luc-linker. The sensitivity of the reporter was assayed as described in the legend to Fig. 1A. Data presented are means ± SD from three independent experiments. **, P < 0.01. EV, empty vector.