Fig 1.

Dengue virus first infects the spleen, followed by bone marrow and then lymph nodes and Peyer's patches after intravenous inoculation. Representative immunohistochemical staining of tissue sections from naïve AG129 mice and AG129 mice at 6, 12, 24, 48, and 72 h after infection (1.5 × 10¹¹ GE of the DENV2 strain E124/128-IC i.v. on day 0). Spleens, cutaneous lymph nodes (CLNs: axillary, brachial, and inguinal lymph nodes), mesenteric lymph nodes (MLNs), Peyer's patches (PPs), and bone marrow (BM) were sectioned and stained for DENV NS3 (white when alone, green when shown with prM/M) and DENV prM/M (antibody clone 2H2, shown in red). The results are representative of 3 mice. WP labels the white pulp, LN labels the center of the lymph nodes, and PP labels the centers of the Peyer's patches.