Table 1.

VSV mutants, cytotoxicity, and fitness in BHK-21 cells

Genome site of substitutiona	Mutated gene	Amino acid substitution	Value (avg ±SEM) for relative:		Type of mutation or referenceb
			Cytotoxicity	Fitness
U906C	N	None	1.100 ± 0.042	1.055 ± 0.021	Random
U1323A	N	F420L	1.153 ± 0.018	0.429 ± 0.024	Random
G1544A	P	R50K	0.972 ± 0.035	1.032 ± 0.016	15
U1820C	P	I142T	0.981 ± 0.042	0.737 ± 0.028	7
G2344A	M	S32N	1.063 ± 0.023	1.032 ± 0.011	18, 29
C2608A	M	P120G	1.072 ± 0.028	0.779 ± 0.017	7
U2617G	M	L123W	1.019 ± 0.030	1.148 ± 0.033	32
A3120U	G	N15Y	0.950 ± 0.022	0.908 ± 0.027	3
A3802G	G	H242R	1.169 ± 0.030	1.160 ± 0.032	7
A4179G	G	T368A	0.838 ± 0.018	0.731 ± 0.019	7
G4459U	G	W461L	0.981 ± 0.026	0.732 ± 0.167	Random
G5597C	L	V289L	0.978 ± 0.025	1.030 ± 0.011	Random
G7128U	L	C799F	0.888 ± 0.036	0.635 ± 0.033	Random
C7287U	L	P852L	0.859 ± 0.026	0.681 ± 0.015	Random
U10809C	L	I2026T	0.997 ± 0.013	0.857 ± 0.014	39
U1323A/G7123C	N/L	F421L/none	1.041 ± 0.030	0.179 ± 0.015	Random
C2969A/U3192C	Intergenic/G	None/Y39H	0.894 ± 0.015	0.571 ± 0.037	Random
C3615A/U3192C	G/G	S180T/Y39H	0.619 ± 0.021	0.071 ± 0.017	Random
U3950G/C7287U	G/L	None/P852L	0.825 ± 0.039	0.225 ± 0.009	Random
G406T/U2617G	N/M	G115C/L123W	1.063 ± 0.088	1.038 ± 0.031	32
U2617G/A3802G	M/G	L123W/H242R	0.925 ± 0.034	1.170 ± 0.026	7, 32

^aNucleotide sites in the plus polarity Indiana genome (GenBank accession no. AM690336).

^bReference describing the substitution that was reproduced in this study.