Fig 1.

IRF7 is sumoylated endogenously in type III EBV LCLs. (A to C) KR4 cells were grown, and cell lysates were collected. (A) Nondenaturing immunoprecipitations were performed with SUMO-1, IRF7, or isotype control (anti-EBV EA-R p85) antibodies. Additional nondenaturing (B) or denaturing (C) immunoprecipitations were performed with SUMO-1 or isotype control antibodies. Western blot analyses were used to detect IRF7 expression. Duplicate experiments are shown. (D and E) BL41 EBV WT and BL41 EBV mut cells were grown, and cell lysates were collected. Denaturing immunoprecipitations were performed with 1 mg of cell lysates with SUMO-1, IRF7, or isotype control antibodies. Western blot analyses were used to detect IRF7 or SUMO-1 expression. Duplicate experiments are represented.