Fig 3.

LMP1-induced sumoylation of IRF7 is dependent on CTAR3. (A) 293T cells were transfected as indicated and cultured for 48 h. Cell lysates were collected, and denaturing immunoprecipitations were performed with c-Myc antibodies. Western blot analyses of the immunoprecipitates and WCLs were used to detect IRF7, sumoylated IRF7, and LMP1. (B) 293T cells were transfected as indicated and cultured for 48 h. Cell lysates were collected, and nondenaturing immunoprecipitations were performed with FLAG antibodies, followed by Western blot analyses as described above. (C) 293T cells were transfected with GFP-IRF7, Myc–SUMO-1, and the indicated FLAG-LMP1 constructs. Cell lysates were collected 48 h posttransfection, and Western blot analyses were used to detect IRF7, sumoylated IRF7, and LMP1. Actin served as a loading control.