Fig 6.

LMP1-induced sumoylation of IRF7 limits its transcriptional activity. (A and B) 293T cells were transfected as indicated along with ISRE-luciferase and beta-galactosidase and assayed 24 h posttransfection. Firefly luciferase activity was normalized to beta-galactosidase expression, and fold changes in ISRE activity were calculated. Data are shown as means ± SD. (C) 293T cells were transfected as indicated, and total RNA was harvested 24 h posttransfection. RT-PCR was carried out to determine IFN-β and GAPDH mRNA levels, and fold changes in IFN-β expression (relative to GADPH expression) were determined. Data are shown as means ± SD.