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Reactivity of antibodies derived from donor-matched EBV+ and EBV− memory B cells to MOG. Reactivity was assessed with a flow cytometry-based assay using an oligodendrocyte cell line expressing MOG. Positive (anti-MOG antibody)- and negative (irrelevant antibody)-control (ctrl) antibody binding is shown by the gray and black bars, respectively. Reactivity is expressed as the ratio of the MFI of staining on MOG+ cells to the MFI of staining on MOG− cells.
