Fig 2.

Synergistic and antagonistic effects of multiple NiV-G N-glycans in combination on cell-cell fusion. (A) Relative levels of 293T cell surface expression (HA), binding to MAbs (MAb26, MAb45, and MAb213), binding to soluble ephrinB2 receptor, and cell-cell fusion. All levels for the mutants were separately normalized to levels seen for wild-type NiV-G, set at 100%. The data shown are averages plus standard deviations of three or more independent experiments. The P value shown for mutant G4G7 was calculated with an unpaired Student t test and multiplied by 12, which takes into account the Bonferroni correction for multiple pairwise comparisons (wild-type NiV-G versus 12 mutants). The axis was broken to aid the visualization of the results for most mutants. (B) Western blot analysis of double N-glycan mutants in 293T cell lysates, displaying their gel mobility differences from the wild-type NiV-G. A representative image out of three independent experiments is shown. (C) Western blot analysis of triple N-glycan mutants in 293T cell lysates, displaying their gel mobility differences from the wild-type NiV-G. A representative image out of three experiments is shown.