Fig 4.

Protein expression of subgenomic Env reporters. 293T cells were transfected with the SA6-env-luc reporter constructs (A) and with the SA5-env-luc reporter constructs (B). Luciferase production was analyzed as described for Fig. 3. Results of 14 measurements are shown (means ± SEM). The mean value is shown next to each bar. Statistical analysis indicated that luciferase production differed significantly between SA6-env-luc (wt) and mutant 4rev (A). There is also a statistically significant difference in luciferase production between SA5-env-luc (wt) and mutant 1234rev (B).