Fig 5.

Northern blot analysis of reporter transcripts. 293T cells were transfected with the wt and mutant Rev-luc (A, C) and Env-luc (B, D) plasmids. (A, B) After 48 h, total cellular RNA was isolated and size-separated on a denaturing gel, and the reporter RNA was visualized with a ³²P-labeled luciferase probe by Northern blotting (control, pGL3-control; mock, pBluescript). (C, D) The 18S and 28S rRNA markers of the Rev-luc transfected cells (C) and the Env-luc transfected cells (D) were identified by ethidium bromide staining and used as a loading control.